MUTYH Deficiency Is Associated with Attenuated Pulmonary Fibrosis in a Bleomycin-Induced Model

Figure 4

MUTYH deficiency alleviated SSB formation and maintained mtDNA integrity. (a, b) Representative images of immunostaining of 8-oxoG and quantification of the intensity of 8-oxoG immunostaining in pulmonary tissue sections ( for per genotype/treatment; scale bar: 100 μm). (c, d) Pulmonary tissue specimens of mice were subjected to immunofluorescence staining with anti-ssDNA (red, original magnification: ×630, , and scale bar: 67 μm). The immune complexes and DAPI-stained nuclei (blue) were visualized under a fluorescence microscope. Total fluorescence intensity of 4 fields selected randomly in 3 sections was quantified with ImageJ (b). (e, f) An 8.7 kb fragment from β-globin loci and a 10 kb fragment from mtDNA were used for measuring nDNA and mtDNA integrity, respectively. At least two independent experiments were performed for each sample (). Statistical significance was analyzed using two-way ANOVA and followed by the LSD post hoc test. represents and # represents compared with the NS-Mutyh^+/+ group. $ represents compared to the BLM-Mutyh^+/+ group. $ represents and & represents compared to the BLM-Mutyh^+/+ group.

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