MUTYH Deficiency Is Associated with Attenuated Pulmonary Fibrosis in a Bleomycin-Induced Model

Figure 6

MUTYH deficiency maintained mitochondrial homeostasis. (a) Representative images of transmission electron microscopy (TEM) of lung AECIIs from different groups of mice (lamellar bodies used as identification marks, orange arrows; green scale bar: 5 μm, ). The boxed region of the image is magnified in the right panel (red scale bar: 1 μm). Mitochondria in AECIIs of pulmonary tissues from model mice were mostly enlarged. The normal mitochondria were indicated with green arrows and dysmorphic ones with red arrows. (b) Quantitative morphometric analyses of the mitochondrial area in single AECII with TEM images. (c) Mitochondrial number per AECII was counted in the TEM images from different groups of mice. (d) Expression levels of the proteins involved in mitochondrial fusion/fission dynamic regulation. Western blotting with anti-MFN2, DRP1, and PINK1 was shown. (e) Histograms show the relative expression levels of MFN2, DRP1, and PINK1 proteins (). Statistical significance was analyzed using two-way ANOVA and followed by the LSD post hoc test. represents and # represents compared with the NS-Mutyh^+/+ group. $ represents and & represents compared to the BLM-Mutyh^+/+ group.

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