The Nrf2/PGC1α Pathway Regulates Antioxidant and Proteasomal Activity to Alter Cisplatin Sensitivity in Ovarian Cancer

Figure 2

Proteasome inhibition promotes nuclear factor E2-related factor 2 (Nrf2) localization to the nucleus through the Kelch-like ECH-associated protein 1 (Keap1)/Nrf2 pathway. (a, b) The nuclear proteins of A2780 and SKOV3 cells were collected after treatment with 100 nM Epox for 6 or 12 h, and Nrf2 levels were measured via Western blotting. Data are presented as the , , compared with the respective controls. A2780 and SKOV3 cells were treated with 100 nM Epox for 12 h. (c) Immunofluorescence staining was used to determine the location of Nrf2 in the nucleus (magnification, ×400). (d) Immunoprecipitation was performed using anti-Nrf2 antibody followed by Western blotting using anti-Nrf2 and anti-Keap1 antibodies. (e) Immunofluorescence staining was used to assess the colocalization of Nrf2 with Keap1 (magnification, ×400).

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