The Nrf2/PGC1α Pathway Regulates Antioxidant and Proteasomal Activity to Alter Cisplatin Sensitivity in Ovarian Cancer

Figure 3

Proteasome inhibition enhances the antioxidant and antiapoptosis activity in SKOV3 cells but not A2780 cells. A2780 and SKOV3 cells were both treated with 100 nM Epox for 12 h. (a) RT-qPCR was used to detect the mRNA expression of GCLC, NQO-1, SOD2, HMOX-1, and G6PDH. Data are presented as the , , , compared with the respective controls. (b) DCFH-DA staining was used to evaluate reactive oxygen species (ROS) levels. (c) Annexin V/PI staining was used to evaluate apoptosis levels after exposure to Epox for 24 h. (d) Relative PPARGC1A mRNA expression was measured by RT-qPCR after exposure to Epox for 12 h. Data are presented as the , , compared with the respective controls. (e) Western blot analysis of peroxisome proliferator-activated receptor-γ coactivator 1α (PGC1α) expression in A2780 and SKOV3 cells. Data are presented as the , , compared with A2780 cells.

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