The Nrf2/PGC1<i>α</i> Pathway Regulates Antioxidant and Proteasomal Activity to Alter Cisplatin Sensitivity in Ovarian Cancer

<div>Nuclear factor E2-related factor 2 (Nrf2) regulates peroxisome proliferator-activated receptor-<i>γ</i> coactivator 1<i>α</i> (PGC1<i>α</i>) at the transcriptional level in SKOV3 cells. (a) Schematic diagram of the sequence spanning from −2000 to +1 relative to the translational start site (TSS) of the PGC1<i>α</i> promoter containing the Nrf2 motif. (b) SKOV3 cells were transfected with Nrf2 or pcDNA3.1 plasmids (vector plasmid as the NC group). Relative <i>NFE2l2</i> mRNA expression was measured by RT-qPCR. Data are presented as the <span class="nowrap"><svg height="9.10327pt" id="M87" style="vertical-align:-0.3499298pt" version="1.1" viewbox="-0.0498162 -8.75334 58.1969 9.10327" width="58.1969pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M797 0V26C739 32 732 36 732 103V296C732 394 682 449 605 449C576 449 550 437 529 423C504 407 475 389 446 366C425 418 382 449 334 449C303 449 279 437 253 421C222 403 201 385 180 371V452C135 432 85 419 41 411V388C99 379 102 374 102 310V103C102 38 93 32 27 26V0H238V26C189 32 180 38 180 103V338C210 363 250 390 289 390C351 390 377 348 377 275V103C377 37 368 32 306 26V0H520V26C465 32 456 38 456 101V296C456 314 455 326 453 338C491 369 529 390 565 390C628 390 653 345 653 274V107C653 36 642 32 583 26V0H797Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.595,0)"><path d="M380 106C343 72 306 56 265 56C195 56 116 112 115 248C235 252 361 262 377 265C396 269 400 277 400 297C400 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(c) The luciferase activities of Epox-treated or Nrf2 overexpression SKOV3 cells transfected with pGL4-PGC1<i>α</i> and <i>Renilla</i> luciferase reporter (pRL-TK) plasmids were determined using dual-luciferase reporter assays. Data are presented as the <span class="nowrap"><svg height="9.10327pt" id="M90" style="vertical-align:-0.3499298pt" version="1.1" viewbox="-0.0498162 -8.75334 58.1969 9.10327" width="58.1969pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M797 0V26C739 32 732 36 732 103V296C732 394 682 449 605 449C576 449 550 437 529 423C504 407 475 389 446 366C425 418 382 449 334 449C303 449 279 437 253 421C222 403 201 385 180 371V452C135 432 85 419 41 411V388C99 379 102 374 102 310V103C102 38 93 32 27 26V0H238V26C189 32 180 38 180 103V338C210 363 250 390 289 390C351 390 377 348 377 275V103C377 37 368 32 306 26V0H520V26C465 32 456 38 456 101V296C456 314 455 326 453 338C491 369 529 390 565 390C628 390 653 345 653 274V107C653 36 642 32 583 26V0H797Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.595,0)"><path d="M380 106C343 72 306 56 265 56C195 56 116 112 115 248C235 252 361 262 377 265C396 269 400 277 400 297C400 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Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: The Nrf2/PGC1<i>α</i> Pathway Regulates Antioxidant and Proteasomal Activity to Alter Cisplatin Sensitivity in Ovarian Cancer 

Oxidative Medicine and Cellular Longevity

The Nrf2/PGC1α Pathway Regulates Antioxidant and Proteasomal Activity to Alter Cisplatin Sensitivity in Ovarian Cancer

Figure 5