Cardioprotective Natural Compound Pinocembrin Attenuates Acute Ischemic Myocardial Injury via Enhancing Glycolysis

Figure 8

Pinocembrin promotes PFKFB3 expression through activating transcription factor HIF1α. (a) Cardiomyocytes were treated with pinocembrin and subsequent to H/R, and then, PFKFB3 expression was detected by qRT-PCR. versus normoxia with the vehicle group. ^# versus H/R with the vehicle group. (b) Cardiomyocytes were transiently cotransfected with a pGL4-basic luciferase expression vector, containing 3,308 bp of the mouse PFKFB3 gene promoter. Cells were exposed to H/R with or without pinocembrin. Transfections were performed in triplicate. versus normoxia with the vehicle group. ^# versus H/R with the vehicle group. (c) Cardiomyocytes were transiently transfected with pGL4-basic luciferase expression vectors containing various lengths of the PFKFB3 gene promoter 5-flanking region, and cells were treated with pinocembrin subsequent to H/R. versus normoxia with the vehicle group. ^# versus H/R with the vehicle group. (d) Transcriptional activity of reporter constructs carrying binding sites for HIF1α transfected in cardiomyocytes insulted with H/R. versus normoxia with the vehicle group. ^# versus H/R with the vehicle group. (e) Analysis of PFKFB3 promoter activity in cardiomyocytes transfected with control, HIF1α-specific siRNA. each. Data represent the . versus corresponding si-NC group.

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