Direct Peritoneal Resuscitation with Pyruvate Protects the Spinal Cord and Induces Autophagy via Regulating PHD2 in a Rat Model of Spinal Cord Ischemia-Reperfusion Injury

Table 1

Primers used in this study.

Name		Nucleotide sequence of primers (5-3)	Size (bp)
H-HIF-1a	Sense	CCGATGGAAGCACTAGACAAAGT	285
	Antisense	TTTGAGGACTTGCGCTTTCAG
H-mTOR	Sense	TCAGCCTGTCAGAATCCAAGTC	196
	Antisense	TTGAAGATGAAGGTGATGGCC
H-BECN1	Sense	GGCACAATCAATAACTTCAGGC	203
	Antisense	GGCAGCTCCTTAGATTTGTCTG
H-LC3II	Sense	TCCGACTTATTCGAGAGCAGC	167
	Antisense	AGCATTGAGCTGTAAGCGCC
H-BNIP3	Sense	CGTTCCAGCCTCGGTTTCTA	287
	Antisense	TATTTTCCGGCCGACTTGAC
H-RHEB	Sense	GACCTGCATATGGAAAGGGTG	199
	Antisense	AGCAGAATCACATCACCGAGC
H-LC3I	Sense	ACCGCTGTAAGGAGGTACAGC	281
	Antisense	GAAGCCGTCCTCGTCTTTCT
H-p62	Sense	GAACAGATGGAGTCGGATAACTG	232
	Antisense	CTGGGAGAGGGACTCAATCAG
H-phd2	Sense	TACGCCACTGTAACGGGAAG	235
	Antisense	GGTTCAATGTCAGCAAACTGG
H-GAPDH	Sense	CATCATCCCTGCCTCTACTGG	259
	Antisense	GTGGGTGTCGCTGTTGAAGTC
R-Rheb	Sense	AGCAGGGCAGGATGAATATTC	161
	Antisense	TAATCGGTATCTGCACTTTCCC
R-MTOR	Sense	TAATGACGAGACCCAGGCTAAG	122
	Antisense	ATGCTCCATCGTGAGTATCCC
R-HIF-1α	Sense	AAGCCCAGAGTCACTGGGACT	118
	Antisense	GTACTCACTGGGACTGTTAGGCTC
R-beclin-1	Sense	TCAATGCGACCTTCCATATCTG	222
	Antisense	CTGTCAGGGACTCCAGATACGAG
R-GAPDH	Sense	CGCTAACATCAAATGGGGTG	201
	Antisense	TTGCTGACAATCTTGAGGGAG
R-LC3-I	Sense	CGAGTTGGTCAAGATCATCCG	138
	Antisense	CCGTCTTCATCCTTCTCCTGTT
R-LC3-II	Sense	GAGTGGAAGATGTCCGGCTC	229
	Antisense	GACACACTCACCATGCTGTGC
R-BNIP3	Sense	ATGGGATTGGTCAAGTCGGC	205
	Antisense	CTTCCAATGTAGATCCCCAAT