Synergism and Antagonism of Two Distinct, but Confused, Nrf1 Factors in Integral Regulation of the Nuclear-to-Mitochondrial Respiratory and Antioxidant Transcription Networks

Figure 10

Nfe2l1 and Nfe2l2 contribute to differential expression of PGC-1α/β and associated genes. (a) The PGC-1α/β-centered interaction network with distinct families of 21 transcription factors and additional 4 cofactors, which was constructed by the STRING tool (https://string-db.org/). (b) The Log2 (fold change) values of those indicated genes were calculated by comparison of RNA-sequencing data obtained from either Nfe2l1α-induced or Nfe2l2-induced cells with equivalent wild-type (WT) controls. (c) Similar Log2 (fold change) values of the above-described genes were also calculated by comparison of RNA-sequencing data obtained from Nfe2l1α^-/-, Nfe2l1α^-/-+siNfe2l2, or Nfe2l2^-/-ΔTA cell lines versus their equivalent WT controls (). (d) A heat map shows differential expression levels of Nfe2l1α-affected genes determined by comparison of either Nfe2l1α^-/- or Nfe2l1α-induced cells with the respective WT controls (). Such different colored genes were scaled by their Log2 values. (e) Another heat map of 19 Nfe2l2-affected genes, which were determined by comparison of Nfe2l2^-/-ΔTA or Nfe2l2-induced cells with their respective WT controls ().

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