Glutamate Attenuates the Survival Property of IGFR through NR2B Containing N-Methyl-D-aspartate Receptors in Cortical Neurons

Figure 5

NR2B antagonist hinders the inhibitory effect of glutamate on the tyrosine phosphorylation of IGF-1R and the survival effect of IGF-1 on cultured cortical neurons. Following pretreatment with Ro25-6981 (1 μM) and NVP-AAM077 (1 μM) for 30 min, 1 mM glutamate was given to cultured cortical neurons. Then, the neurons were exposed to 100 ng/ml IGF-1 for 8 min (for tyrosine phosphorylation of IGF-1R) and 48 h (for survival assay). The tyrosine phosphorylation of the IGF-1R, densitometric analysis (a), and cell viability (b) were determined as described in Experimental Treatments. Ro25-6981 blocked the effect of glutamate on the tyrosine phosphorylation of the IGF-1R (a) and protected cortical neurons from glutamate’s excitotoxicity (b). Blots represent prototypical examples of experiments replicated at least three times. Data represent assays from at least three independent experiments. .

(a)

(b)