Opuntiol Prevents Photoaging of Mouse Skin via Blocking Inflammatory Responses and Collagen Degradation

Figure 5

Opuntiol prevents MAPK, NF-κB, and AP-1 expressions in UVA (100 J/cm²)-irradiated mouse skin. (a) Western blot analysis of opuntiol (50 mg/kg b.wt.) on UVA-linked tp-ERK1, p-JNK, and p-p38 and its total protein expression in mouse skin. (b) The bar diagram represents the quantification of protein which was measured by Image Studio software (LI-COR). The phosphorylated form of MAPK proteins was normalized by total protein of MAPKs, and β-actin was used to ensure the equal volume of protein loaded. (c) Western blot analysis of opuntiol on UVA-linked translocation of NF-κB and AP-1 expression in mouse skin. The translocation of NF-κB and AP-1 expression was analyzed by cytosolic and nuclear extract of protein samples. (d) The bar diagram represents the quantification of proteins which was analyzed by Image Studio software (LI-COR). β-Actin was used to normalize the examined protein and to ensure the equal volume of protein loaded The data represent from three independent experiments, and values not sharing a common marking ( and #) differ significantly at (Duncan’s multiple range test (DMRT)).

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