Melatonin Attenuates Calcium Deposition from Vascular Smooth Muscle Cells by Activating Mitochondrial Fusion and Mitophagy via an AMPK/OPA1 Signaling Pathway

Table 1

Real-time qPCR primers.

Gene	Forward primer (5->3)	Reverse primer (5->3)
MMP9	AATCTCTTCTAGAGACTGGGAAGGAG	AGCTGATTGACTAAAGTAGCTGGA
MIP1α	CTGCCCTTGCTGTCCTCCTCTG	CTGCCGGCTTCGCTTGGTTA
IL-8	TCCTAGTTTGATACTCCCAGTC	ACAAGTTTCAACCAGCAAGA
CII-30	CCTCTAGATACCGATAGCC	AACTTACGATAGGCTGATCCG
CIII-core2	CTCTAGGAATCCGATAGTCTA	GGCAAGTAGATACCAGTA
CIV-II	GAGGGTCCTAGATCCGAT	GTTGACCAGACCATAGTCCAT
Drp1	AGACCTCTCATTCTGCAACTG	TTACCCCATTCTTCTGCTTCC
Fis1	TGTCCAGTCCGTAACTGAC	TTCGATACCTGACTTAC
Mfn2	ATGTGGCCCAACTCTAAGTG	CACAAACACATCAGCATCCAG
Mfn1	ACCTGTTTCTCCACTGAAGC	TGGCTATTCGATCAAGTTCCG
Beclin1	GGCAGTGGCTCCTATT	GGCGTGCTGTGCTCTGAAAA
P62	CTCGCTATGGCGTCGCTCACCGTG	TCACTCTGGCGGGAGATGTGGGTA
Runx2	CACTGGCGGTGCAACAAGA	TTTCATAACAGCGGAGGCATTTC
β-Actin	GATGGTGGGTATGGGTCAGAAGGAC	GCTCATTGCCGATAGTGATGACT