MiR-429 Involves in the Pathogenesis of Colorectal Cancer via Directly Targeting LATS2

Figure 3

Sh-miR-429 inhibits tumorigenesis both in vitro and in vivo. (a) Knockdown of miR-429 in RKO and HCT116 cell lines by short hairpin RNA technology. (b, c) Knockdown of miR-429 reduced the colony formation ability of RKO and HCT116 cells. (d) Forced expressions of miR429 in RKO and HCT116 cell lines. (e) Overexpression of miR429 increased the colony formation ability of RKO and HCT116 cells. (f, g) sh-miR-429 reduced tumor growth rates of RKO and HCT116 xenografts in nude mice (/group). (h) shRNA interfering knock down the expression levels of miR-429 in the xenografts. (i) sh-miR-429 reduced tumor weights and dimensions of RKO and HCT116 xenografts in nude mice (/group).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)