MiR-429 Involves in the Pathogenesis of Colorectal Cancer via Directly Targeting LATS2

Figure 4

miR-429 directly targets LATS2 mRNA. (a) Alignment of has-miR-429 and the 3-UTR sequences of LATS2 and the mutant miR429 binding sequence in the 3-UTR of LATS2. (b) Dual-luciferase reporter assay showed the direct interaction between miR429 and LATS2. (c, d) The mRNA levels of LATS2 but not LATS1 in RKO and HCT116 cells reduced by overexpression of miR-429. (e–g) Western blotting analysis confirmed that LATS2 but not LATS1 was downregulated by overexpression of miR-429. (h) Western blotting analysis confirmed that LATS2 protein expression levels were retrieved in the xenografts of sh-miR-429.

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