Research Article
Selenium-Enriched Yeast Alleviates Oxidative Stress-Induced Intestinal Mucosa Disruption in Weaned Pigs
Table 2
Primer sequences used for quantitative RT-PCR.
| | Gene | Accession no. | Primer sequences (5-3) | Product length (bp) |
| | Nrf2 | XM_003133500.5 | GCCCCTGGAAGCGTTAAAC | 67 | | GGACTGTATCCCCAGAAGGTTGT |
| | HO-1 | NM_001004027 | CGCTCCCGAATGAACAC | 112 | | GCTCCTGCACCTCCTC |
| | Bcl-2 | XM_021099593.1 | GCTACTTACTGCCAAAGGGA | 110 | | TTCAGGCGGAGCTGTAAGAG |
| | Caspase-3 | NM_214131.1 | GGAATGGCATGTCGATCTGGT | 105 | | ACTGTCCGTCTCAATCCCA |
| | SGLT1 | NM_001164021.1 | CGCGTCCGGTGTGAAAG | 137 | | CTTCCCGATATCTACACATTCCA |
| | GLUT2 | NM_001097417.1 | GAGGCAGCAGTAGGGAATCTTCGAGCA | 140 | | ACAGTTACTCTGACACCCGTTCTTC |
| | β-Actin | XM_003124280.5 | TGGAA CGGTG AAGGT GACAGC | 177 | | GCTTTTGGGAAGGCAGGGACT |
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Nrf2: nuclear factor E2-related factor 2; HO-1: heme oxygenase-1; Bcl-2: B-cell lymphoma-2; caspase-3: cysteine aspartic protease-3; SGLT1: sodium glucose transport protein-1; GLUT2: glucose transporter-2.
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