Hypoxia-Induced Glioma-Derived Exosomal miRNA-199a-3p Promotes Ischemic Injury of Peritumoral Neurons by Inhibiting the mTOR Pathway

Figure 3

HIGDE aggravated OGD injury in normal HT22 cells, and miRNA-199a-3p was identified as a target miRNA that induced the different hypoxic injury effects between HIGDE and NHIGDE. (a) Exosomes were isolated from C6 glioma cells and were identified by TEM. Isolated exosomes were consistent in size and shape. (b) The expression of CD81 was clearly detected in isolated HIGDE/NHIGDE and C6 cells. (c) After applying OGD/reperfusion injury, the LDH level in HT22 cells was significantly increased with HIGDE treatment in comparison to NHIGDE and vehicle. vs. no OGD; ^# vs. NHIGDE and vehicle. (d) After applying OGD/reperfusion injury, the LDH level of HT22 cells treated with HIGDE+RNase was lower than that in cells treated with HIGDE+vehicle but was similar as to that in cells treated with NHIGDE+vehicle. vs. NHIGDE+vehicle; ^## vs. HIGDE+vehicle. (e) MicroRNA microarray analysis indicated that among the sixteen miRNAs that were expressed significantly higher in HIGDE than NHIGDE, the most upregulated one was miRNA-199a-3p (, fold ). (f) Confirmed by RT-qPCR, the expression of miRNA-199a-3p was almost two times higher in HIGDE than NHIGDE. vs. NHIGDE. /group in at least 3 independent experiments.

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