Hypoxia-Induced Glioma-Derived Exosomal miRNA-199a-3p Promotes Ischemic Injury of Peritumoral Neurons by Inhibiting the mTOR Pathway

Figure 4

miRNA-199a-3p could aggravate OGD injury in primary cultured neurons. (a) RT-qPCR confirmed that the constructed miRNA-199a-3p mimics were successfully transformed into primary cultured neurons. vs. NC (premiRNA negative control). (b) After OGD/reperfusion injury, the LDH level of primary cultured neurons treated with NHIGDE+miRNA-199a-3p mimics was higher than that in cells treated with NHIGDE+vehicle but was similar to that in cells treated with HIGDE+vehicle. vs. NHIGDE+vehicle. (c) RT-qPCR validated that the expression of miRNA-199a-3p in HIGDE from C6 cells was knocked down by miRNA-199a-3p shRNA. vs. scramble shRNA. (d) After OGD/reperfusion injury, the LDH release level by primary cultured neurons treated with HIGDE+miRNA-199a-3p shRNA or NHIGDE was lower than that of primary cultured neurons treated with HIGDE+scramble shRNA. vs. NHIGDE; ^# vs. HIGDE+scramble shRNA. /group in at least 3 independent experiments.

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