Yolkin Isolated from Hen Egg Yolk as a Natural Immunoregulator, Activating Innate Immune Response in BMDM Macrophages

Figure 8

Effect of MAPK inhibitors on yolkin-mediated iNOS expression (a) and activation (b). BMDM cells were preincubated with MAPK inhibitors: U0126 for ERK and SP600125 for JNK for 2 h, followed by treatment with yolkin. Cells were lysed and expression of iNOS was determined by Western blot using monoclonal anti-iNOS antibodies (a). Supernatants were used to determine NO concentration by the Griess reaction (b). Results represent three independent experiments and present (). compared with the control, and and compared with yolkin.

(a)

(b)