lncRNA 00312 Attenuates Cell Proliferation and Invasion and Promotes Apoptosis in Renal Cell Carcinoma via miR-34a-5p/ASS1 Axis

Figure 4

miR-34a-5p negatively regulates the function of lncRNA 00312. (a) Luciferase reporter assay indicated miR-34a-5p is the target of lncRNA 00312. (b) Efficacy of miR-34a-5p mimic was detected via RT-PCR analysis (). (c) miR-34a-5p mimic promoted proliferation and inhibited function of lncRNA 00312 in A498 and ACHN cell lines (). NC mimic: cells transfected unrelated RNA as negative control; NC inhibitor: cells transfected empty vector of miR-34a-5p inhibitor as negative control. (d, e) miR-34a-5p mimic attenuated effect on promoting apoptosis of lncRNA 00312 and further inhibited cell apoptosis by TUNEL staining () and western blot analysis (). (f) Invasion of RCC cells was determined after lncRNA 00312 and transfection of miR-34a-5p mimic (). NC: cells transfected nonsense sequence and empty vector as negative control. , , and versus respective control (multiple comparison: NC group versus lncRNA 00312 group; ^$NC group versus miR-34a-5p mimic group; ^&lncRNA 00312 group versus lncRNA+miR-34a-5p mimic group).

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