lncRNA 00312 Attenuates Cell Proliferation and Invasion and Promotes Apoptosis in Renal Cell Carcinoma via miR-34a-5p/ASS1 Axis

Figure 5

miR-34a-5p inhibitor diminished function of inhibiting lncRNA 00312. (a) qRT-PCR analysis revealed efficacy of miR-34a-5p inhibitor (). NC inhibitor: cells transfected empty vector as negative control. (b) miR-34a-5p inhibitor blocks lncRNA 00312 siRNA pro-proliferation in RCC cell lines (). NC: cells transfected empty vector as negative control. (c, d) TUNEL staining () and Western blot analysis () exhibited miR-34a-5p inhibitor which can partially block the inhibition of lncRNA 00312 siRNA on apoptosis in two RCC cells. NC: cells transfected empty vector as negative control. (e) miR-34a-5p inhibitor could reverse the invasive effect of inhibiting lncRNA 00312 (). NC: cells transfected empty vector as negative control. , , and versus respective control (multiple comparison: NC group versus si-lncRNA 00312 group; ^$NC group versus miR-34a-5p inhibitor group; ^&si-lncRNA 00312 group versus si-lncRNA 00312+miR-34a-5p inhibitor group).

(a)

(b)

(c)

(d)

(e)