lncRNA 00312 Attenuates Cell Proliferation and Invasion and Promotes Apoptosis in Renal Cell Carcinoma via miR-34a-5p/ASS1 Axis

Figure 6

lncRNA 00312 performed its function via ASS1. (a) RT-PCR analysis determined efficacy of interfering ASS1 (). NC: cells transfected two empty vectors as negative control. (b) Interference of ASS1 could increase cell proliferation and inhibited function of lncRNA 00312 (). NC: cells transfected two empty vectors as negative control. (c, d) Inhibiting ASS1 diminished the effect on promoting apoptosis of lncRNA 00312 and further inhibited cell apoptosis by TUNEL staining () and Western blot analysis (). NC: cells transfected two empty vectors as negative control. (e) ASS1 inhibition increased cell invasion and attenuated the effect of lncRNA 00312 inhibiting cell invasion (). , , and versus respective control (multiple comparison: NC group versus lncRNA 00312 group; ^$NC group versus si-ASS1 group; ^&lncRNA 00312 group versus lncRNA 00312+si-ASS1 group). NC: cells transfected nonsense sequence as negative control. (f) Western blot analysis result for ASS1 expression. lncRNA 00312+: cells transfected lncRNA 00312 overexpression vector; lncRNA 00312-: cells transfected empty vector of lncRNA 00312 overexpression vector; ASS1 expression +: cells transfected ASS1 overexpression plasmid; ASS1 expression -: cells transfected empty si-ASS1 overexpression plasmid.

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