miR-155-5p Promotes Oxalate- and Calcium-Induced Kidney Oxidative Stress Injury by Suppressing MGP Expression

Figure 5

MGP is a direct target of miR-155-5p in HK-2 cells. (a) Schematic diagram of the mutant and wild-type seed sequences of miR-155-5p targeting the 3-UTR of MGP. (b, d) Western blot and qRT-PCR analyses were performed to detect the protein expression of MGP after miR-155-5p inhibitor and mimics treatment in HK-2 cells. (c) qRT-PCR analysis was performed to detect the expression level of miR-155-5p in HK-2 cells. (e) Luciferase reporters harbouring putative target sites in the WT and mutant 3-UTR of MGP were cotransfected with miR-155-5p mimics or inhibitor in HK-2 cells. and vs. NC group.

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