Quadrella incana (Capparaceae) Leaf Extract Enhances Proliferation and Maintenance of Neural Stem/Progenitor Cells through Upregulating Glycolytic Flux and Redox Potential

Figure 2

Quadrella incana leaf extract did not suppress the differentiation of NSPCs in the differentiating condition. (a) Immunofluorescence analysis of βIII-tubulin (green) expression in DMSO- or Quadrella incana leaf extract-treated and differentiated NSPCs. Nuclear DAPI (4,6-diamidino-2-phenylindole) staining is in blue. . (b) Western blot analysis of βIII tubulin in NSPCs upon vehicle or Quadrella incana leaf extract treatment under proliferating condition (prol) or differentiating condition (diff). The relative band intensity of βIII tubulin protein is shown on the graph below. (c) mRNA expression of Tubb3 in NSPCs upon vehicle or 100 μg/ml Quadrella incana leaf extract treatment under proliferating condition (prol) or differentiating condition (diff) was determined by quantitative real-time PCR (qRT-PCR). Values are . , , .

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