Quadrella incana (Capparaceae) Leaf Extract Enhances Proliferation and Maintenance of Neural Stem/Progenitor Cells through Upregulating Glycolytic Flux and Redox Potential

Figure 4

Quadrella incana leaf extract improved the oxidation-reduction potential of NSPCs by upregulating ROS scavenging genes. (a) Western blot analysis of FoxO1, FoxO3, and Fubp1 in NSPCs kept under proliferating media after treatment for 3 days with DMSO, Quadrella incana leaf extract, or six different plant crude extracts. β actin was used as a loading control. (b) Western blot analysis of Fubp1, FoxO1, and Sox2 in proliferating NSPCs after treatment with DMSO, 20 μg/ml or 100 μg/ml Quadrella incana leaf extract for 3 days. Expression of each protein was normalized to that of GAPDH. The relative band intensity of each protein is shown in the graph below. For each protein, the intensity of the DMSO-treated sample was arbitrarily set as 1. (c) Relative mRNA expression levels of Fubp1 and FoxO1 in proliferating NSPCs treated with DMSO, 20 μg/ml or 100 μg/ml Quadrella incana leaf extract for 3 days. Values are . (d) Western blot analysis of Prdx3 in proliferating NSPCs after treatment with DMSO or 100 μg/ml Quadrella incana leaf extract for 3 days. The relative band intensity of Prdx3 protein is shown in the graph below. The intensity of DMSO-treated sample was arbitrarily set as 1. (e) mRNA expression analysis of SOD2 in proliferating NSPCs treated with DMSO or 100 μg/ml Quadrella incana leaf extract for 3 days. Values are . (f) Relative ratio of reduced to oxidized glutathione (GSH/GSSG) in NSPCs treated with DMSO or 100 μg/ml Quadrella incana leaf extract.

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