Protective Effect of Quercetin against H₂O₂-Induced Oxidative Damage in PC-12 Cells: Comprehensive Analysis of a lncRNA-Associated ceRNA Network

Figure 3

Distinct expression pattern of mRNAs and functional enrichment analysis. The MA plot of DE mRNA expression profiles (a) between the CON group and the model group and (b) between the model group and the QUE group. The volcano plot of DE mRNA expression profiles (c) between the CON group and the model group and (d) between the model group and the QUE group. Unsupervised clustering analysis showing the expression profiles of the top 20 upregulated and downregulated mRNAs (e) between the CON group and the model group and (f) between the model group and the QUE group. (g) Heat map showing 294 mRNAs that were changed when PC-12 cells were subjected to oxidative damage; pretreatment with QUE reversed these alterations. (h) GO enrichment analysis of mRNAs altered by QUE. The superscripted number represents the number of genes annotated in the GO term, the ordinate represents the GO term, and the abscissa represents the −log₁₀( value). (i) KEGG enrichment analysis of mRNA altered by QUE. The ordinate represents the KEGG pathway, and the abscissa represents the adjusted value. CON: control; DE: differentially expressed; GO: Gene Ontology; H₂O₂: hydrogen peroxide; KEGG: Kyoto Gene and Genomic Encyclopedia; MOL: model; mRNA: messenger RNA; QUE: quercetin.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)