Protective Effect of Quercetin against H₂O₂-Induced Oxidative Damage in PC-12 Cells: Comprehensive Analysis of a lncRNA-Associated ceRNA Network

Figure 4

Distinct expression pattern of lncRNAs and functional enrichment analysis. The MA plot of the expression profiles of DE lncRNAs (a) between the CON group and the model group and (b) between the model group and the QUE group. The volcano plot of the expression profiles of DE lncRNAs (c) between the CON and model groups and (d) between the model and QUE groups. Unsupervised clustering analysis showing the expression profiles of the top 20 upregulated and downregulated lncRNAs (e) between the CON and model groups and (f) between the model and QUE groups (f). (g) Heat map showing 197 lncRNAs that were altered when PC-12 cells were subjected to oxidative damage; pretreatment with QUE reversed these alterations. (h) GO enrichment analysis of targeted genes for lncRNAs altered by QUE. The superscripted number represents the number of genes annotated in the GO term, the ordinate represents the GO term, and the abscissa represents the −log₁₀( value). (i) KEGG enrichment analysis of targeted genes for lncRNAs altered by QUE. The ordinate represents the KEGG pathway, and the abscissa represents the adjusted value. CON: control; DE: differentially expressed; GO: Gene Ontology; H₂O₂: hydrogen peroxide; KEGG: Kyoto Gene and Genomic Encyclopedia; lncRNA: long noncoding RNA; MOL: model; QUE: quercetin.

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