Protective Effect of Quercetin against H₂O₂-Induced Oxidative Damage in PC-12 Cells: Comprehensive Analysis of a lncRNA-Associated ceRNA Network

Figure 6

Differential expression of ncRNAs and mRNAs validated by real-time quantitative polymerase chain reaction (qPCR). The data showed that the expression levels of the lncRNAs (MSTRG.4076.1, MSTRG.48686.2, and MSTRG.34031.1), miRNAs (novel_miR_97, novel_miR_298, novel_miR_2218, novel_miR_1502, and novel_miR_2117), and mRNA (PLK2, MDM2, IL6, GADD45G, and CDKN1A) were upregulated in H₂O₂-induced PC-12 cells and downregulated when pretreated with QUE. The expression levels of MSTRG.89940.1 were downregulated in H₂O₂-induced PC-12 cells and upregulated when pretreated with QUE. The real-time qPCR results were consistent with the RNA sequencing data. , (a) H₂O₂ vs. CON; (b) QUE vs. H₂O₂. QUE represents H₂O₂-induced PC-12 cells pretreated with QUE. CON represents untreated PC-12 cells. H₂O₂ represents H₂O₂-induced PC-12 cells. CON: control; H₂O₂: hydrogen peroxide; lncRNA: long noncoding RNA; mRNA: messenger RNA; miRNA: microRNA; ncRNA: noncoding RNA; QUE: quercetin.

(a)

(b)