Research Article
Protective Effect of Quercetin against H2O2-Induced Oxidative Damage in PC-12 Cells: Comprehensive Analysis of a lncRNA-Associated ceRNA Network
Table 1
Primers designed for qRT-PCR validation of candidate miRNAs, mRNAs, and lncRNAs.
| | Names | Forward primer and reverse primer | Tm (°C) | Product length (bp) |
| | ACTB | F: 5 CGAGTACAACCTTCTTGCAGC3
R: 5 ACCCATACCCACCATCACAC3 | 60 | 202 | | β-Actin (R) | F: 5CGAGTACAACCTTCTTGCAGC3
R: 5 ACCCATACCCACCATCACAC3 | 60 | 202 | | U6 | F: 5GCTTCGGCAGCACATATACTAAAAT3
R: 5CGCTTCACGAATTTGCGTGTCAT3 | 60 | 89 | | IL6 | F: 5 TCAGAGCAATACTGAAACCCTA3
R: 5 TCCTTAGCCACTCCTTCTGT3 | 60 | 134 | | PLK2 | F: 5 ATGGTGGCGATCTCCCTAGT3
R: 5 AGCGAACAGCCAGACATCAA3 | 60 | 247 | | MDM2 | F: 5 TGAGGTCTATCGGGTCACAGTC3
R: 5 CAGGCACATCTAAGCCTTCTTCT3 | 60 | 263 | | CDKN1A | F: 5 TCTTGTGATATGTACCAGCCACAG 3
R: 5 GTCAAAGTTCCACCGTTCTCG 3 | 60 | 182 | | GADD45G | F: 5 GTCTACGAGTCCGCCAAAGTC3
R: 5 CTATGTCGCCCTCATCTTCT3 | 60 | 92 | | MSTRG.89940.1 | F: 5 GTCACCTTACCGTAGGCACA 3
R: 5 CCAAATTCCTCCAGCTTCACT 3 | 60 | 157 | | MSTRG.4076.1 | F: 5 AGTGCTCCCGACATTCACTT 3
R: 5 TCCTTACGCTCAATCTATCCC 3 | 60 | 103 | | MSTRG.48686.2 | F: 5 GCAGTACAACAATTATCGCCAA 3
R: 5 AGGTCCTTAGAGTGAGCAACG 3 | 60 | 197 | | MSTRG.34031.1 | F: 5 TTTCCACAGCCTTTCCACG 3
R: 5 CACTTCCACATTGCTCTTCATC 3 | 60 | 196 | | Novel_miR_1502 | GSP: 5GGGGAATACTGGGTGCTGT3
R: 5GTGCGTGTCGTGGAGTCG3 | 60 | 64 | | Novel_miR_2218 | GSP: 5GGGGTTCTGGGTGCTGTA3
R: 5GTGCGTGTCGTGGAGTCG3 | 60 | 62 | | Novel_miR_298 | GSP: 5GAAACGGCGGCGATG3
R: 5GTGCGTGTCGTGGAGTCG3 | 60 | 59 | | Novel_miR_345 | GSP: 5GGAGGAGGGAACGCAGTCT3
R: 5GTGCGTGTCGTGGAGTCG3 | 60 | 64 | | Novel_miR_97 | GSP:5GGGGGTGTCTGTCTGAGTG3
R: 5GTGCGTGTCGTGGAGTCG3 | 60 | 62 |
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