Generation of Cellular Reactive Oxygen Species by Activation of the EP2 Receptor Contributes to Prostaglandin E2-Induced Cytotoxicity in Motor Neuron-Like NSC-34 Cells

Figure 3

Effects of NAC pretreatment on PGE2- and EP agonist-induced cell death detected by MTT reduction assay. Differentiated NSC-34 cells were exposed to various concentrations of NAC for 4 h and then treated with vehicle (0.15% DMSO), 80 μM PGE2, 40 μM butaprost (an EP2 agonist), or 40 μM sulprostone (an EP1/3 agonist) for 48 h. The viability of the cells was assessed by the MTT reduction assay. Results are expressed as percentages relative to the control (nontreated) cells. Values represent for four separate experiments. , , .