Generation of Cellular Reactive Oxygen Species by Activation of the EP2 Receptor Contributes to Prostaglandin E2-Induced Cytotoxicity in Motor Neuron-Like NSC-34 Cells

Figure 4

Effect of NAC pretreatment on PGE2- and EP agonist-induced LDH leakage from differentiated NSC-34 cells. Differentiated NSC-34 cells were exposed to various concentrations of NAC for 4 h, and then the cells were treated with vehicle (0.15% DMSO), 80 μM PGE2, 40 μM butaprost (an EP2 agonist), or 40 μM sulprostone (an EP1/3 agonist) for 48 h. After exposure, the amount of LDH released into the medium was assayed as described in Experimental Procedures. Graphs show the relative levels of LDH in these cells. Values are calculated as percentages of LDH released relative to that of cells treated with 0.2% Tween-20. Values represent for four separate experiments. , , .