Generation of Cellular Reactive Oxygen Species by Activation of the EP2 Receptor Contributes to Prostaglandin E2-Induced Cytotoxicity in Motor Neuron-Like NSC-34 Cells

<div>Phase-contrast photomicrographs of differentiated NSC-34 cells treated with PGE2 and EP agonists. Differentiated NSC-34 cells were treated with vehicle (0.15% DMSO), 80 <i>μ</i>M PGE2, 40 <i>μ</i>M butaprost (an EP2 agonist), or 40 <i>μ</i>M sulprostone (an EP1/3 agonist) in the presence or absence of NAC for 48 h. Cell morphology was observed using a phase-contrast microscope (IX71, Olympus, Tokyo, Japan). Photographs show typical phase-contrast microscopy images in each treatment group. Scale bar indicates 50 <i>μ</i>m.</div>

Oxidative Medicine and Cellular Longevity

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Figure 5

Figure 5: Generation of Cellular Reactive Oxygen Species by Activation of the EP2 Receptor Contributes to Prostaglandin E2-Induced Cytotoxicity in Motor Neuron-Like NSC-34 Cells 

Figure 5 | Generation of Cellular Reactive Oxygen Species by Activation of the EP2 Receptor Contributes to Prostaglandin E2-Induced Cytotoxicity in Motor Neuron-Like NSC-34 Cells 