Generation of Cellular Reactive Oxygen Species by Activation of the EP2 Receptor Contributes to Prostaglandin E2-Induced Cytotoxicity in Motor Neuron-Like NSC-34 Cells

<div>LIVE/DEAD staining of differentiated NSC-34 cells treated with PGE2 and EP agonists. Differentiated NSC-34 cells were treated with vehicle (0.15% DMSO), 80 <i>μ</i>M PGE2, 40 <i>μ</i>M butaprost (an EP2 agonist), or 40 <i>μ</i>M sulprostone (an EP1/3 agonist) in the presence or absence of NAC for 48 h. Photographs show typical fluorescence images of calcein-AM (green, live cells) and EthD-1 (red, dead cells) double staining in each treatment group. Scale bar indicates 50 <i>μ</i>m. Graphs show the percentage of EthD-1-positive dead cells in these cells. 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Oxidative Medicine and Cellular Longevity

fig6

Figure 6

Figure 6: Generation of Cellular Reactive Oxygen Species by Activation of the EP2 Receptor Contributes to Prostaglandin E2-Induced Cytotoxicity in Motor Neuron-Like NSC-34 Cells 

Figure 6 | Generation of Cellular Reactive Oxygen Species by Activation of the EP2 Receptor Contributes to Prostaglandin E2-Induced Cytotoxicity in Motor Neuron-Like NSC-34 Cells 