Role of Nrf2 in Lipopolysaccharide-Induced Acute Kidney Injury: Protection by Human Umbilical Cord Blood Mononuclear Cells

Figure 3

The changes of blood parameters and renal damages in intervention experiment. We observed the changes of SCr, BUN, body temperature, MAP, and apoptosis of treated rats in the control group, LPS group, LPS+MNCs group, and LPS+MNCs+ML385 group. (a) Changes of SCr level in different groups. (b) Changes of BUN level in different groups. (c) Changes of body temperature level in different groups. (d) Changes of MAP level in different groups. (e) Representative photomicrographs of tubular cell injury in rat kidney tissue sections of the control group, LPS group, LPS+MNCs group, and LPS+MNCs+ML385 group (original magnifications: ×200; hematoxylin and eosin stain). (f) Semiquantitative analysis of histologic scoring. (g) Representative blots of cleaved caspase 3 and GAPDH. (h) Densitometry of cleaved caspase 3. (i) Representative pictures of TUNNEL assay (×200). Rat kidney tissue was stained for TUNEL (green). Nuclei were stained with DAPI (blue). (j) Apoptosis percentage. Greater than 200 cells in each group were evaluated to determine the percentage of TUNEL-positive cells. Values are . ^★ versus the control group. ^# versus the LPS group. ^∆ versus the LPS+MNCs group.

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