Research Article
Role of Nrf2 in Lipopolysaccharide-Induced Acute Kidney Injury: Protection by Human Umbilical Cord Blood Mononuclear Cells
Figure 4
The changes of ROS, NLRP3, Keap1, Nrf2, HO-1, and HMGB1 in intervention experiment. Western blot was used to detect the expression of NLRP3, Keap1, Nrf2, HO-1, and HMGB1. Oxidative stress in kidney tubular cells was assessed using dihydroethidium (DHE). (a) Representative blots of NLRP3, Keap1, Nrf2, HO-1, HMGB1, and β-tubulin. (b) Densitometry of NLRP3. (c) Densitometry of Keap1. (d) Densitometry of Nrf2. (e) Densitometry of HO-1. (f) Densitometry of HMGB1. (g) Representative images of DHE staining. Original magnifications: ×200. (h) Semiquantitative analysis of DHE fluorescence intensity. Values are , for each group. ★ versus the control group. # versus the LPS group. ∆ versus the LPS+MNCs group.
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