AVE0991, a Nonpeptide Angiotensin 1-7 Receptor Agonist, Improves Glucose Metabolism in the Skeletal Muscle of Obese Zucker Rats: Possible Involvement of Prooxidant/Antioxidant Mechanisms

Figure 9

Protein tyrosine phosphatase 1B (PTP1B) in the skeletal muscle of obese Zucker rats. Protein content of total protein tyrosine phosphatase 1B (PTP1B), phosphorylated PTP1B at Ser50 with representative Western blots, and PTP1B activity in musculus quadriceps of control obese Zucker rats and in obese Zucker rats receiving AVE0991 via osmotic minipumps for two weeks. The amount of protein was quantified by Western blot method, and PTP1B activity was measured using commercially available colorimetric assay kit. The signal intensities of the proteins of interest were normalized to the sample’s total protein content stained with Coomassie Brilliant Blue. Data are presented as . Results were analysed by Student’s -test, ; ; .