Oxidative Medicine and Cellular Longevity

Research Article

AVE0991, a Nonpeptide Angiotensin 1-7 Receptor Agonist, Improves Glucose Metabolism in the Skeletal Muscle of Obese Zucker Rats: Possible Involvement of Prooxidant/Antioxidant Mechanisms

Table 1

Primer sequences used for qPCR.


Ace	Fw	5-ATG GTA CAG AAG AAG GGC TGG AA-3
	Rv	5-TTG TAG AAG TCC CAC GCA GA-3

Ace2	Fw	5-TCA GAG CTG GGA TGC AGA AA-3
	Rv	5-GGC TCA GTC AGC ATG GAG TTT-3

Agt	Fw	5-CAT GAG TTC TGG GTG GAC AA-3
	Rv	5-AAG TTG TTC TGG GCG TCA CT-3

Apa (Enpep)	Fw	5-GGC TCC CTT GTG GGT TTT TAC-3
	Rv	5-TCT TGT TGG GTT CAT CGA AAC A-3

AT₁ (Agtr1a)	Fw	5-TCT CAG CAT CGA TCG CTA CCT-3
	Rv	5-AGG CGA GAC TTC ATT GGG TG-3

AT₂	Fw	5-ACC TTT TGA ACA TGG TGC TTT G-3
	Rv	5-GTT TCT CTG GGT CTG TTT GCT C-3

Furin	Fw	5-GGG TTT CCC AGC AGT CTT CA-3
	Rv	5-GCC AGA TCC CCA GGT GTG-3

Mas1 (MasR)	Fw	5-TGA CCA TTG AAC AGA TTG CCA-3
	Rv	5-TGT AGT TTG TGA CGG CTG GTG-3

Nep (Mme)	Fw	5-GCA GAA ATC AGA TCG TCT TCC CCG-3
	Rv	5-CTG AGT CCA CCA GTC AAC GAG GT-3

Nox2	Fw	5-TGA TCA TCA CAT CCT CCA CCA A-3
	Rv	5-GAT GGC AAG GCC GAT GAA-3

Nox4	Fw	5-CTGCATCTGTCCTGAACCTCAA-3
	Rv	5-TCTCCTGCTAGGGACCTTCTGT-3

Nrf2	Fw	5-GTT GAG AGC TCA GTC TTC AC-3
	Rv	5-CAG AGA GCT ATC GAG TGA CT-3

p22^phox	Fw	5-TGGCCTGATCCTCATCACAG-3
	Rv	5-AGGCACGGACAGCAGTAAGT-3

Plzf (Zbtb16)	Fw	5-GCGAAGAAGAAGAGGACCGTAAG-3
	Rv	5-CCGGAATGCTTCGAGATGAA-3

Renín	Fw	5-CCA CCT TCA TCC GCA AGT TC-3
	Rv	5-TGC GAT TGT TAT GCC GGT C-3

Rer (Atp6ap2)	Fw	5-TGG CCT ATA CCA GGA GAT CG-3
	Rv	5-AAT AGG TTG CCC ACA GCA AG-3

Rps29	Fw	5-GCTGAACATGTGCCGACACT-3
	Rv	5-GGTCGCTTAGTCCAACTTAATGAA-3

Sod1	Fw	5-CAC TCT AAG AAA CAT GGC G-3
	Rv	5-CTG AGA GTG AGA TCA CAC G-3

Sod2	Fw	5-TTC AGC CTG CAC TGA AG-3
	Rv	5-GTC ACG CTT GAT AGC CTC-3

Sod3	Fw	5-CTT GAC CTG GTT GAG AAG ATA G-3
	Rv	5-GAT CTG TGG CTG ATC GG-3