Neuroprotective Effect of Salvianolic Acid A against Diabetic Peripheral Neuropathy through Modulation of Nrf2

Figure 5

Salvianolic acid A attenuated oxidative stress in high glucose-injured RSC96 Schwann cells. (a) In vitro DPPH assay. Fresh DPPH and different concentrations of SalA were incubated at room temperature for 30 min. The absorbance value at 515 nm was recorded by a SpectraMax M5. RSC96 cells were treated with 150 mM glucose and different concentrations of SalA for 24 h. Intracellular ROS (b), ABTS (TEAC) (c), GSSG (d), MDA (e), and SOD activity (f) were measured using the corresponding kits. All data are presented as , . , , and , compared with the HG group. (g) qPCR analysis of the Sod1, Sod2, and Gpx1 gene expression in RSC96 cells treated with 150 mM glucose and different concentrations of SalA for 24 h. All qPCR data are normalized to TBP and presented as , . , , and , compared with the HG group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)