Neuroprotective Effect of Salvianolic Acid A against Diabetic Peripheral Neuropathy through Modulation of Nrf2

Figure 7

Salvianolic acid A improved the mitochondrial function in high glucose-injured RSC96 Schwann cells. RSC96 cells were treated with 150 mM glucose and different concentrations of SalA for 24 h. (a) Representative images showing fluorescence of mitochondrial superoxide were detected by MitoSOX™ Red staining (scale  μm). (b) Mitochondrial membrane potential was examined after the cells were incubated with fluorescent dye Rhodamine 123 for 10 min. The fluorescence intensity (excitation 488 nm, emission 535 nm) was detected using SpectraMax M5 microplate reader. (c) Mitochondrial morphology visualized by staining with MitoTracker Green FM was examined using a laser scanning microscope (scale  μm). (d) Intracellular ATP level was determined by a CellTiter-Glo® kit. All data are presented as , . , , and , compared with the HG group. (e) qPCR analysis of the Cox8b, Sdhb, Atp5j, and Uqcrc1 gene expression in RSC96 cells treated with 150 mM glucose and different concentrations of SalA for 24 h. All qPCR data are normalized to TBP and presented as , . and , compared with the HG group.

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