Hyperglycemia Induces Endoplasmic Reticulum Stress in Atrial Cardiomyocytes, and Mitofusin-2 Downregulation Prevents Mitochondrial Dysfunction and Subsequent Cell Death

Figure 5

Decreasing ER-mitochondria interactions by genetic downregulation of mitofusin-2 (Mfn2) protects HL-1 cells from mitochondria dysfunction. (a) Flow cytometry detected HL-1 cells that stained with DCFH-DA. (b) Quantification of ROS by DCFH-DA intensity in (a). Data represent the ( independent experiments). (c, d) JC-1 staining. The ratio of red/green fluorescence reflects changes in the mitochondrial membrane potential of HL-1 cells. Data represent the ( independent experiments). Scale bar, 100 μm. (e, f) Analysis of oxygen consumption rate (OCR) from a Seahorse XF 24 Extracellular Flux Analyzer. Oligomycin inhibits ATP synthase, FCCP uncouples oxygen consumption from ATP production, and AA+Rotenone inhibits complexes I and III, respectively. Data represent the ( independent experiments), ^#.

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