Human Tissue Kallikrein 1 Improves Erectile Dysfunction of Streptozotocin-Induced Diabetic Rats by Inhibition of Excessive Oxidative Stress and Activation of the PI3K/AKT/eNOS Pathway

Figure 1

Verification of the existence and expression of hKLK1 and rKLK1 genes in the corpus cavernosum. Panel (a) shows representative hKLK1 genomic DNA bands in the corpus cavernosum through conventional polymerase chain reaction followed by agarose gel electrophoresis. Panel (b) shows relative mRNA expression of the hKLK1 to β-actin within the corpus cavernosum of different groups by real-time reverse transcriptase polymerase chain reaction. Data are shown as with six samples per group. Panels (c) and (d) show rKLK1 and hKLK1 protein expressions in the corpus cavernosum by western blot with five samples per group. Panel (e) shows bradykinin level in all groups. Each bar represents . , , and compared to WTR group. compared to WTDM group. hKLK1: human tissue kallikrein 1; rKLK1: rat tissue kallikrein 1; WTR: wild-type rats; TGR: transgenic rats; WTDM: diabetic wild-type rats; TGDM: diabetic transgenic rats; TGDMH: diabetic transgenic rats administrated with HOE140.

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