Human Tissue Kallikrein 1 Improves Erectile Dysfunction of Streptozotocin-Induced Diabetic Rats by Inhibition of Excessive Oxidative Stress and Activation of the PI3K/AKT/eNOS Pathway

Figure 5

hKLK1 could activate PI3K/AKT/eNOS signaling in the corpus cavernosum of diabetic rats. Panels (a) and (b) show protein expressions of PI3K(p55), PDE5, and ICa-L normalized to β-actin level, and panels (c), (d), and (e) show the ratios of pAKT/AKT, PeNOS(S1177)/eNOS, and PeNOS(T495)/eNOS to evaluate their activity in all groups. Panels (f) and (g) show NO and cGMP levels normalized to total protein concentration of penile samples. Each bar represents . , , and . hKLK1: human tissue kallikrein 1; NO: nitric oxide; cGMP: cyclic guanosine monophosphate; WTR: wild-type rats; TGR: transgenic rats; WTDM: diabetic wild-type rats; TGDM: diabetic transgenic rats; TGDMH: diabetic transgenic rats administrated with HOE140.

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