Human Tissue Kallikrein 1 Improves Erectile Dysfunction of Streptozotocin-Induced Diabetic Rats by Inhibition of Excessive Oxidative Stress and Activation of the PI3K/AKT/eNOS Pathway

Table 1

Primers used in conventional PCR and real-time RT-PCR.

Genes	Primer sequences	Usage (PCR)
hKLK1	F: 5-GTCCAGAAGGTGACAGACTTCAT-3; R: 5-GTCCTCGATCCACTTCACATAAG-3	Conventional
	F: 5-CTCACAGCTGCTCATTGCATC-3; R: 5-GCTCTCACTGACATGAACAAACTGG-3	Real time
rKLK1	F: 5-CCCACACACAGATGGTGACAGA-3; R: 5-CCTTGAAGCACACCATCACAGAG-3	Real time
β-Actin	F:5-AAGAGCTATGAGCTGCCTGA-3; R: 5-TACGGATGTCAACGTCACAC-3	Conventional and real time

PCR = polymerase chain reaction; hKLK1 = human tissue kallikrein 1; rKLK1 = rat tissue kallikrein 1.