G9a Suppression Alleviates Corneal Neovascularization through Blocking Nox4-Mediated Oxidative Stress

Figure 2

Angiogenesis depended on oxidative stress in HUVECs under the H/R model. Cultured HUVECs were treated with NAC or DMSO under H/R condition, and NAC(1 mM) or DMSO was added 10 min prior to H/R. The expression of VEGF (a,b) was detected by western blot. Serum-starved HUVECs were seeded onto the Matrigel (c,d) and incubated after H/R with or without NAC treatment. ROS production (e) and H₂O₂ production (f) were measured after treatment with NAC under H/R. The mRNA levels of proangiogenic factors were also determined by quantitative real-time PCR (g-l). Data are expressed as (). versus control; versus H/R. Experiments were repeated 3 times.

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