G9a Suppression Alleviates Corneal Neovascularization through Blocking Nox4-Mediated Oxidative Stress

Figure 7

Treatment with BIX 01294 reduced angiogenesis and oxidative stress induced by alkali burn in the cornea. Western blot was performed for the expression of G9a and VEGF (a-c) after treatment with BIX (20 mg/kg, once daily) and quantification of their expression in fold change relative to the control group. SOD activity (d), MDA content (e), and H2O2 production (f) were also detected after treatment with BIX. The mRNA levels of proangiogenic factors were also determined by quantitative real-time PCR (g-l). Data are expressed as (). versus control; ^# versus alkali burn. Experiments were repeated 3 times.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)