The MUC5B Mucin Is Involved in Paraquat-Induced Lung Inflammation

Figure 1

PQ-induced mouse acute lung injury (ALI) activated the NF-κB pathway and induced MUC5B expression. (a, b) Static compliance and resistance of the lung. (c) Quantification of lavaged bronchoalveolar lavage (BAL) cells after PQ instillation. (d, e) Levels of the proinflammatory cytokines TNF-α and IL-6 in both BALF and serum. (f, g) phospho-NF-κB p65 from protein level of mice lung tissue. (h) MUC5B mRNA levels of lung tissue were measured by RT-qPCR. (i) Mouse lung sections stained with hematoxylin and eosin (HE). Original magnification: ×200. Scale bar represents 100 μm. (j) IHC staining with Muc5b antibody of injured lung tissues from control or paraquat-treated mice. Original magnification: ×200. Scale bar represents 100 μm. ^&, , , and compared to the saline control. , , , PQ groups compared between day 3 and day 7. Saline group (), PQ group () in both day 3 and day 7 experiments.

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