The MUC5B Mucin Is Involved in Paraquat-Induced Lung Inflammation

Figure 5

MUC5B mRNA expression and TNF-α and IL-6 release were decreased via MAPK and NF-κB pathway inhibition in PQ-induced A549 cells. Cells were preincubated with various concentrations of MAPK and NF-κB pathway inhibitors for 2 h and then treated with 50 μM PQ for 30 min. Total RNA was analyzed by qRT-PCR. TNF-α and IL-6 levels in the cell culture supernatants were analyzed by ELISA. Inhibiting the MAPK-NF-κB signaling pathway by inhibitors (a) BAY, (b) SCH, (c) SP, and (d) SB all decreased the expression of MUC5B and the extracellular factors TNF-α and IL-6 at 24 h after treatment with 50 μM PQ. The data are shown as the . of three different experiments. , , , compared with the no-treatment group; , , , compared with the PQ group.

(a)

(b)

(c)

(d)