Shen Shuai II Recipe Attenuates Apoptosis in 5/6 Renal Ablation/Infarction Rats by Inhibiting p53 and the Mitochondrial Pathway of Apoptosis

Figure 3

SSR inhibited the mitochondrial accumulation of proapoptotic Bax and Puma proteins in the CKD model. (a) Representative immunoblot demonstrating reduced translocation of cytochrome c (Cyt c) 8 weeks after SSR treatment in 5/6 (A/I) rats. Gapdh and VDAC1 were used as loading controls. (b) Quantification of the relative protein levels of Cyt c in the mitochondria and cytosol (). (c) Representative immunoblot showing mitochondrial Bax and Puma protein content. (d) Quantification of mitochondrial Bax and Puma levels (). (e) The protein levels of complex II (SDHB) and complex V (ATPB) subunits were determined by immunoblotting. (f) Quantification of SDHB and ATPB levels (). (g) The level of the mitochondrial copy number was determined by the ratio of the cytochrome c oxidase subunit I (COX I) gene to β-actin (). Values are . , .

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