Sodium Tanshinone IIA Sulfonate Attenuates Erectile Dysfunction in Rats with Hyperlipidemia

Figure 6

Activation of the Nrf2/HO-1 pathway and antioxidant capacity in STS-pretreated CSMC. (a) Cell viability of STS-treated CSMC. Cells were treated with STS (0-60 μg/ml) for 48 hours, and cell viability was measured by a CCK8 assay. (b) Cell viability of H₂O₂-treated CSMC. Cells were treated with H₂O₂ (50 μM, 100 μM, 200 μM, and 400 μM) for 6, 12, and 18 hours, and cell viability was measured. (c) Effect of STS pretreatment on the Nrf2/HO-1 pathway in H₂O₂-treated CSMC. The CSMC were treated with 100 μM H₂O₂ for 12 hours with or without 2 hours of STS (1 μg/ml, 5 μg/ml, and 10 μg/ml) pretreatment, and the expression of Nrf2 and HO-1 was determined by Western blot. (d, e) Effect of STS pretreatment on Nrf2 translocation in H₂O₂-treated CSMC. (f) Expression of HO-1 was measured by Western blot. (g, i) Effect of STS pretreatment on antioxidant capacity in H₂O₂-treated CSMC. The CSMC were treated with 100 μM H₂O₂ for 12 hours with or without 2 hours of 10 μg/ml STS pretreatment, and the MDA, GSH, and SOD levels were measured. Each bar represents of three independent experiments; per group. compared to the NC group; compared to the NC group; ^# compared with the H₂O₂ group; ^## compared with the H₂O₂ group.

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