| | | Primary antibody | Species/types | Dilution | Catalogue number | Product information | | H2S-generating enzyme markers | CSE | Mouse mAb | IF 1 : 50
WB 1 : 2000 | 60234-1-Ig | Proteintech, Rosemont, IL, USA | | CBS | Rabbit pAb | IF 1 : 50
WB 1 : 2000 | 14787-1-AP | Proteintech, Rosemont, IL, USA | | 3-MST | Rabbit pAb | WB 1 : 500 | NBP1-82617 | Novus Biologicals, Centennial, CO, USA | | Antioxidant enzyme markers | Nrf2 | Rabbit pAb | WB 1 : 1000 | ab137550 | Abcam, Cambridge, MA, USA | | HO-1 | Rabbit pAb | IF 1 : 100
WB 1 : 1500 | 27282-1-AP | Proteintech, Rosemont, IL, USA | | Smooth muscle markers | α-SMA | Rabbit pAb | IF 1 : 50 | 14395-1-AP | Proteintech, Rosemont, IL, USA | | Calponin | Mouse mAb | IF 1 : 50 | sc-58707 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
| | | Secondary antibody | Types | Dilution | Catalogue number | Product information | | FITC conjugate | Goat anti-mouse | IgG (H+L) | IF 1 : 50 | SA00003-1 | Proteintech, Rosemont, IL, USA | | Goat anti-rabbit | IgG (H+L) | IF 1 : 50 | SA00003-2 | Proteintech, Rosemont, IL, USA | | Cy3 conjugate | Goat anti-mouse | IgG (H+L) | IF 1 : 50 | SA00009-1 | Proteintech, Rosemont, IL, USA | | Goat anti-rabbit | IgG (H+L) | IF 1 : 50 | SA00009-2 | Proteintech, Rosemont, IL, USA |
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