Tetrastigma hemsleyanum Vine Flavone Ameliorates Glutamic Acid-Induced Neurotoxicity via MAPK Pathways

Figure 3

Effect of THVF on Glu-induced mitochondrial dysfunction in PC12 cells (). (a) Mitochondrial membrane potential, mitochondrial membrane lipid peroxidation, and GSH alterations of PC12 cells in the presence of Glu and THVF under different treatments and were incubated with RH123, NAO, and NDA probes. (b) The quantitative data of panel (a). Images were captured with a fluorescence microscope in the same settings. All the fluorescence images were quantified in the whole field with the background removed and represented by normalized fluorescence (-axes) via Image-Pro Plus 6.0 (). Significance analysis was carried out according to one-way ANOVA test and different letters in figures mean statistically significant differences among the groups (a, b, c, etc., were labeled from large to small and once columns containing a same word means statistically insignificant, otherwise means statistically significant, ).

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