Tetrastigma hemsleyanum Vine Flavone Ameliorates Glutamic Acid-Induced Neurotoxicity via MAPK Pathways

Figure 4

THVF treatment altered expressions of proteins in PC12 cells. (a) Western blot bands of Bax, Bcl-2, caspase-9, PCNA, and β-actin (). (b) Western blot bands of MAPKs. p-JNK, p-p38, and p-ERK represent phosphorylation of JNK, p38, and ERK. (c) Relative expressions of Bax, Bcl-2, caspase-9, PCNA, p-JNK, p-p38, p-ERK, JNK, p38, and ERK. The images were quantified by ImageJ software, the intensity of bands was corrected by β-actin (), and vertical lines in the histogram represent SDs of three replicates. Significance analysis was carried out according to the one-way ANOVA test, and different letters mean statistically significant differences among the groups (a, b, c, etc., were labeled from large to small and once columns containing a same word means statistically insignificant, otherwise means statistically significant, ).

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